ChemBioScreen
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PLATEFORMES


Criblage Biologique et Chimiothèques

CBCMise à jour le 03/05/2017



Responsable


Hélène Munier-Lehmann

Thématique principale


Anti-infectieux

Techniques spécifiques


Variées (tests cellulaires et biochimiques), HTS sur protéines purifiées, cultures cellulaires ou pathogènes.

Systemes de détection


Lecteurs microplaques (SunriseTM, Infinite® M200 and Infinite® M1000 Pro) pour absorbance, luminescence, fluorescence (intensité, polarisation, HTRF), AlphaScreen®/AlphaLISA®


Equipements particuliers


Dans un laboratoire de niveau de sécurité P2, deux plateformes robotiques TECAN avec systèmes de pipettage, bras robotiques, carroussels, incubateurs et lecteurs intégrés, situées sous des plafonds soufflants pour des conditions stériles lorsque nécessaire.
Gestion des chimiothèques et des données de criblage par un LIMS dédié et développé à façon par la société Modul-Bio.


Taille de la chimiothèque


73000 molécules

Brevets / Publications / Communications


Publications (2011-2016) :

1          Grandin, C., Hourani, M. L., Janin, Y. L., Dauzonne, D., Munier-Lehmann, H., Paturet, A., Taborik, F., Vabret, A., Contamin, H., Tangy, F. and Vidalain, P. O. (2016) Respiratory syncytial virus infection in macaques is not suppressed by intranasal sprays of pyrimidine biosynthesis inhibitors. Antiviral Research. 125, 58-62

2          Laskaris, P., Atrouni, A., Calera, J. A., d'Enfert, C., Munier-Lehmann, H., Cavaillon, J. M., Latge, J. P. and Ibrahim-Granet, O. (2016) Administration of zinc chelators improves survival of mice infected with Aspergillus fumigatus both in monotherapy and in combination with caspofungin. Antimicrobial Agents and Chemotherapy. 60, 5631-5639

3          Raj, S., Krishnan, K., Askew, D. S., Helynck, O., Suzanne, P., Lesnard, A., Rault, S., Zeidler, U., d'Enfert, C., Latge, J. P., Munier-Lehmann, H. and Saveanu, C. (2016) The Toxicity of a Novel Antifungal Compound Is Modulated by Endoplasmic Reticulum-Associated Protein Degradation Components. Antimicrob Agents Chemother. 60, 1438-1449

4          Lucas-Hourani, M., Munier-Lehmann, H., El Mazouni, F., Malmquist, N. A., Harpon, J., Coutant, E. P., Guillou, S., Helynck, O., Noel, A., Scherf, A., Phillips, M. A., Tangy, F., Vidalain, P. O. and Janin, Y. L. (2015) Original 2-(3-Alkoxy-1H-pyrazol-1-yl)azines Inhibitors of Human Dihydroorotate Dehydrogenase (DHODH). J. Med. Chem. 58, 5579-5598

5          Munier-Lehmann, H., Lucas-Hourani, M., Guillou, S., Helynck, O., Zanghi, G., Noel, A., Tangy, F., Vidalain, P. O. and Janin, Y. L. (2015) Original 2-(3-Alkoxy-1H-pyrazol-1-yl)pyrimidine Derivatives as Inhibitors of Human Dihydroorotate Dehydrogenase (DHODH). J. Med. Chem. 58, 860-877

6          Vidalain, P. O., Lucas-Hourani, M., Helynck, O., Tangy, F. and Munier-Lehmann, H. (2015) [Stimulation of the antiviral innate immune response by pyrimidine biosynthesis inhibitors: a surprise of phenotypic screening]. Medecine sciences : M/S. 31, 98-104

7          Lucas-Hourani, M., Munier-Lehmann, H., Helynck, O., Komarova, A., Despres, P., Tangy, F. and Vidalain, P. O. (2014) High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses. J. Vis. Exp. 87, e51222

8          Aulner, N., Danckaert, A., Rouault-Hardoin, E., Desrivot, J., Helynck, O., Commere, P. H., Munier-Lehmann, H., Spaeth, G. F., Shorte, S. L., Milon, G. and Prina, E. (2013) High Content Analysis of Primary Macrophages Hosting Proliferating Amastigotes: Application to Anti-leishmanial Drug Discovery. PLoS Negl. Trop. Dis. 7, e2154

9          Carre, C., Jacquier, C., Bouge, A. L., de Chaumont, F., Besnard-Guerin, C., Thomassin, H., Pidoux, J., Da Silva, B., Chalatsi, E., Zahra, S., Olivo-Marin, J. C., Munier-Lehmann, H. and Antoniewski, C. (2013) AutomiG, a Biosensor to Detect Alterations in miRNA Biogenesis and in Small RNA Silencing Guided by Perfect Target Complementarity. PloS one. 8, e74296

10        Lucas-Hourani, M., Dauzonne, D., Jorda, P., Cousin, G., Lupan, A., Helynck, O., Caignard, G., Janvier, G., Andre-Leroux, G., Khiar, S., Escriou, N., Despres, P., Jacob, Y., Tangy, F., Munier-Lehmann, H. and Vidalain, P. O. (2013) Inhibition of Pyrimidine Biosynthesis Pathway Suppresses Viral Growth through Innate Immunity. PLoS Pathog. 9, e1003678

11        Lucas-Hourani, M., Lupan, A., Desprès, P., Thoret, S., Pamlard, O., Dubois, J., Guillou, C., Tangy, F., Vidalain, P. O. and Munier-Lehmann, H. (2013) A phenotypic assay to identify chikungunya virus inhibitors targeting the non-structural protein nsP2. Journal of Biomolecular Screening. 18, 172-179

12       Munier-Lehmann, H., Vidalain, P. O., Tangy, F. and Janin, Y. L. (2013) On dihydroorotate dehydrogenases and their inhibitors and uses. J. Med. Chem. 56, 3148-3167

13        Zeidler, U., Bougnoux, M. E., Lupan, A., Helynck, O., Doyen, A., Garcia, Z., Sertour, N., Clavaud, C., Saveanu, C., Munier-Lehmann, H. and d'Enfert, C. (2013) Synergy of the antibiotic colistin with echinocandin antifungals in Candida species. J. Antimicrob. Chemother. 68, 1285-1296

14        Clavaud, C., Beauvais, A., Barbin, L., Munier-Lehmann, H. and Latgé, J.-P. (2012) The composition of the culture medium influences the β-1,3-glucan metabolism of Aspergillus fumigatus and the antifungal activity of inhibitors of β-1,3-glucan synthesis tested. Antimicrobial Agents and Chemotherapy. 56, 3428-3431

 

Brevet (2011-2016) :

1          Janin, Y. L., Guillou, S., Lucas-Hourani, M., Munier-Lehmann, H., Noel, A., Salanouve, E., Tangy, F. and Vidalain, P. O. (2014) Pyrazole derivatives as dihydroorotate dehydrogenase (DHODH) inhibitors. EP14 305 516.8.