ChemBioScreen
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PLATEFORMES


BAC-SCREEN

Plateforme de criblage de petites molécules sur bactériesMise à jour le 09/03/2018



Responsable


V. Sinou

Thématique principale


Bactériologie, Antibiorésistance, Drug-design

Techniques spécifiques


Cribles in cellulo (HCS), Tests fonctionnels de mesure de la perméabilité & du potentiel membranaires, de l'activité des transporteurs impliqués dans l'antibiorésistance (pompes d'efflux et porines) et de la formation de biofilms 

Systemes de détection


Lecteurs microplaques absorbance, luminescence, fluorescence

Equipements particuliers


Plateforme robotique intégrée, Sécurité microbiologique niveau 2, Souchothèque, Sondes membranaires

Taille de la chimiothèque


1000

Autres labellisations


Plateformes Technologiques du site d'Aix-Marseille (en cours de renouvellement)

Affiliation


Université Aix-Marseille, IRBA, INSERM U1261

Brevets / Publications / Communications


Brevets 
• Berti L, Bolla J-M, Brunel J-M, Casanova J, Lorenzi V. « Use of polyaminoisoprenyl derivatives in antibiotic or antiseptic treatment »: Extension PCT déposée le 23/02/2012 (PCT/EP2012/053113). Licensed by Vibiophen (Labège, France) in 2016.
• Franceschi C, Pinet E, Pagès J-M, Zambardi G. « Identification des bactéries résistantes aux carbapénèmes par Imperméabilité membranaire ». 2015.

Déclaration d’invention 
• « Novel biocides on the electromodulable phosphonium conducting polymers » (Partenariat CiNaM, UMR_MD1).

Publications
• Fankam AG et al. Antibacterial activities of selected Cameroonian spices and their synergistic effects with antibiotics against multidrug-resistant phenotypes. BMC Complement Altern Med. 2011;11:104.
• Voukeng IK et al. Antibacterial and antibiotic-potentiation activities of the methanol extract of some cameroonian spices against Gram-negative multi-drug resistant phenotypes. BMC Res Notes. 2012;5:299.
• Fadli M et al. Antibacterial activity of Thymus maroccanus and Thymus broussonetii essential oils against nosocomial infection-bacteria and their synergistic potential with antibiotics. Phytomedicine. 2012;19:464.
• Goemaere E et al. New PDF-Inhibitors and cooperative interaction : a combination to improve antibacterial activity. J Antimicrob Agents. 2012;67:1392.
• Lacmata ST et al. Antibacterial Activities of Selected Cameroonian Plants and Their Synergistic Effects with Antibiotics against Bacteria Expressing MDR Phenotypes. Evid Based Complement Alternat Med. 2012;2012:623723.
• Fadli M et al. Thymus maroccanus essential oil, a membranotropic compound active on Gram-negative bacteria and resistant isolates. J Appl Microbiol. 2012;113:1120.
• Lieutaud A et al. Inhibitors of Antibiotic Efflux by AcrAB-TolC in Enterobacter aerogenes. Anti-Infect Agents. 2013;11:168.
• Handzlik J et al. Search for new tools to combat Gram-negative resistant bacteria among amine derivatives of 5-arylidenehydantoin. Bioorg Med Chem. 2013;21:135.
• Brunel J-M et al. Polyamino geranic derivatives as new chemosensitizers to combat antibiotic resistant Gram-negative bacteria. Bioorg Med Chem. 2013;21:1174.
• Fadli M et al. Natural extracts stimulate membrane-associated mechanisms of resistance in Gram-negative bacteria. Lett Appl Microbiol. 2014;58:472.
• Fadli M et al. First evidence of antibacterial and synergistic effects of Thymus riatarum essential oil with conventional antibiotics. Ind Crops Prod. 2014;61:370.
• Pieri C et al. New ianthelliformisamine derivatives as antibiotic enhancers against resistant gram-negative bacteria. J Med Chem. 2014;57:4263.
• Allam A et al. New peptides with metal binding abilities and their use as drug carriers. Bioconjug Chem. 2014;25:1811.
• Philippe N et al. In vivo evolution of bacterial resistance in two cases of Enterobacter aerogenes infections during treatment with imipenem. PLoS ONE. 2015;10(9):e0138828.
• Pagès J-M et al. The role of the outer membrane and porins in the susceptibility of β-lactamase-producing Enterobacteriaceae to ceftazidime-avibactam. Antimicrob Agents Chemother. 2015;60:1349.
• Otrebska-Machaj E et al. Efflux pump blockers in Gram-negative bacteria: new hydantoin based modulators to improve antibiotic activity. Frontiers Microb. 2016;7:622.
• Borselli D et al. Polyamino-isoprenic derivatives block intrinsic resistance of P. aeruginosa to doxycycline and chloramphenicol in vitro. PLoS ONE. 2016;11(5):e0154490.
• Brunel F et al. Antibacterial activities of fluorescent nano assembled triphenylamine phosphonium ionic liquids. Bioorg Med Chem Lett. 2016. pii: S0960-894X(16)30540-6. 
• Fadli M et al. Artemisia herba-alba Asso and Cymbopogon citratus (DC.) Stapf essential oils and their capability to restore antibiotics efficacy. Ind Crops Prod. 2016;89:399.
• Fieulaine S et al. A unique peptide deformylase platform to rationally design and challenge novel active compounds. Sci Reports 6. 2016; ID 35429. doi:10.1038/srep35429.
• Borselli D et al. Motuporamine Derivatives as antimicrobial agents and antibiotic enhancers against resistant gram-negative bacteria. Chem Biochem. 2017;18:276.